Effect of lupeol on insulin receptor substrate-1 and AKt expression in adipose tissue of type-2 diabetic rats.

Dhivyadharshini, A.; Vishnpriya, V.; Ponnulakshmi, R.; Gayathri, R.; Madhan, K.; Shyamaladevi, B.; Selvaraj, J.

Background: Lupeol a natural herbal drug is abundant in edible vegetables, fruits, and also in medicinal plants. Adipose tissue is the primary site of storage for excess energy as triglycerides. Hence, the present study was conducted or the beneficial role of lupeol on insulin signaling molecules in adipose tissue of high-fat diet and sucrose-induced type 2 diabetic experimental rats. Objectives: The study aimed to identify the effect of lupeol on insulin receptor substrate-1 (IRS-1) and AKt expression in adipose tissue of type-2 diabetic rats. Materials and Methods: In the present study, experimental animals were divided into four groups each consisting of five animals: Group1: Normal control, Group 2: Diabetic rates (type2), Group 3: Diabetes rates treated with standard with lupeol at the dose of 25 mg/kg b. wt/day/orally for 30 days, and Group 4: Type-2 diabetic rats treated with metformin (50 mg/kg, b. wt/day orally for 30 days). At the day of 30, the animals will be anesthetised by sodium thiopentone, adipose tissue was dissected out from control and treated animals and asked for protein isolation and will be used for the further studies. Results: A significant decrease in IRS-1 mRNA expression in adipose tissue was observed in type-2 diabetic animals, whereas lupeol treatment increased IRS-1 mRNA level in the type-2 diabetic animals. A significant decrease in Akt mRNA levels in adipose tissue was observed in type-2 diabetic animals, whereas lupeol treatment increased Akt protein level in the type-2 diabetic animals as that of the standard drug metformin. Conclusion: It is concluded from the present findings that lupeol has antidiabetic activity through the regulation of insulin signaling molecules such as IRS-1 and Akt mRNA expression in adipose tissue.

ADIPOSE tissues; STREPTOZOTOCIN; INSULIN receptors; INSULIN regulation; HIGH-fat diet; VETERINARY drugs

Drug Invention Today, 2019, Vol 12, Issue 4, p801

0975-7619

Academic Journal