Hepatoprotective effect of lupeol is mediated through gluconeogenic enzymes: An experimental study.

Gajapriya, M.; Selvaraj, J.; Vishnpriya, V.; Ponnulakshmi, R.; Gayathri, R.; Madhan, K.; Shyamaladevi, B.

Aim: This study aims to the aspect of lupeol on gluconeogenic enzymes such as fructose-1,6-bisphosphatase and glucose-6- phosphatase in the liver of type-2 diabetic male rats. Experimental Design: In the present study, healthy adult male albino rats were used and divided four groups each consisting of six animals. The oral effective dose of lupeol (2, orally for 30 days) was administrated to high-fat diet-I plus type 2 diabetic rats. At the end of the experimental period, liver tissues from the control and experimental group were dissected out and used for the assessment of gluconeogenic enzymes (glucose-6-phosphate, fructose-1, 6-bisphosphate, and phosphoenolpyruvate carboxykinase using standard method of spectrophotometric analysis, the data will be subjected to one-way analysis of variance using computer-based software (GraphPad Prism, resin 5). The significance will be considered at the level of P < 0.05. Results: Diabetic rats showed a significant increase in the levels of gluconeogenic enzymes such as fructose-1,6-bisphosphatase and glucose-6-phosphatase in the liver. However, lupeol administration to type-2 diabetic rats significantly reduced the altered levels of the enzymes equal to that of the standard drug metformin. Conclusion: It is concluded from the present findings that lupeol regulates glucose homeostasis in liver through the controlling of the gluconeogenic enzymes as a result of increase in the insulin sensitivity, and hence, lupeol has protective role in hepatocytes.

STREPTOZOTOCIN; ONE-way analysis of variance; ENZYMES; INSULIN resistance; GLUCOSE-6-phosphatase

Drug Invention Today, 2019, Vol 12, Issue 4, p798

0975-7619

Academic Journal