Lupeol normalizes high-fat diet-induced alternations in the Akt levels in the quadriceps muscle of type 2 diabetic rats.

Nirubama, K.; Vishnpriya, V.; Ponnulakshmi, R.; Gayathri, R.; Madhan, K.; Shyamaladevi, B.; Selvaraj, J.

Background: Lupeol is a pharmacologically active triterpenoid found in mango and also in variety of medicinal plants including Acacia visco and Abronia villosa. It is also found in dandelion coffee. Many scientific studies have shown that lupeol possesses many beneficial pharmacological activities including anti-inflammatory, antioxidant, and antihyperglycemic activity. Biomarkers for oxidative damage to DNA, lipids, and proteins are also supporting the concept of increased oxidative stress in diabetes and diabetic nephropathy. In the present study, we elucidated the antioxidant potential of lupeol by analyzing the enzymic and non-enzymic antioxidant in the liver of high-fat diet- and sucrose-induced Type-2 diabetic rats. Objectives: The study aimed to identify the effects of lupeol on Akt and its phosphorylation in the quadriceps muscle of high-fat diet- and sucrose-induced Type-2 diabetic adult male rats. Materials and Methods: Lupeol was administered orally to Type-2 diabetic rats (25 mg/kg body weight) for a period of 30 days. After the treatment period, animals were anesthetized and liver from control and experimental rats dissected out and the used for the studied. Results: There was a marked decrease observed in the levels of Akt and its phosphorylation (p-AktSer473) in the quadriceps muscle of the type-2 diabetic rats. However, lupeol treatment efficiently increased the level of Akt protein compared to diabetic group. Metformin showed a similar pattern to that of lupeol. Conclusion: The present finding concludes that the lupeol regulates insulin-mediated Akt signaling in gracilis muscle. Therefore, the plant-derived triterpenoid may be used as antidiabetic phytocompound for the type-2 diabetic management.

QUADRICEPS muscle; STREPTOZOTOCIN; RATS; DNA damage; DIABETIC nephropathies; CULTIVARS

Drug Invention Today, 2019, Vol 12, Issue 4, p794

0975-7619

Academic Journal