Effect of lupeol on glucose transporter 2 and insulin receptor on high-fat-fed Type 2 diabetic rats.

Akshaya, R.; Vishnpriya, V.; Ponnulakshmi, R.; Gayathri, B.; Madhan, K.; Shyamaladevi, B.; Selvaraj, J.

Background: Lupeol is pharmacologically active triterpenoid. It is also known as Fagarasterol and lupenol. It occurs in the skin of lupin seeds as well as in the latex of fig trees and of rubber plants and was also found in many edible fruits and vegetables. Objectives: The study aimed to identify the effect of lupeol on glucose transporter 2 (GLUT2) and insulin receptor (IR) on high-fat-fed Type 2 diabetic rats. Materials and Methods: In the present study, experimental animals were divided into four groups each consisting of five animals: Group 1: Normal control, Group 2: Diabetic rats (Type 2), Group 3: Diabetic rats treated with standard with lupeol at the dose of 25 mg/kg b.wt/day/orally for 30 days, and Group 4: Type 2 diabetic rats treated with metformin (50 mg/kg b.wt/day orally for 30 days). At the day of 30, the animals will be anesthetized by sodium thiopentone. Liver tissue was dissected out from control and treated animals and used for the assessment of protein expression analysis. Results: A significant decrease in IR was observed in Type 2 diabetic animals when compared to control rats. Conversely, GLUT2 protein expression was found to be increased in Type 2 diabetic rats. Oral administration of lupeol for 30 days significantly restored the altered levels of the insulin signaling molecules in the liver of Type 2 diabetic rats. Conclusion: It is concluded from the present findings that lupeol has antidiabetic activity through the controlling insulin signaling molecules such as IR and GLUT2 protein expression in hepatocytes.

GLUCOSE transporters; INSULIN receptors; METFORMIN; CATABOLITE repression; RATS; RUBBER plants

Drug Invention Today, 2019, Vol 12, Issue 4, p777

0975-7619

Academic Journal