Neuroprotective efficacy of withaferin a on aging mediated oxidative stress in striatum and Substantia nigra of wistar albino rat.

Banu, Mohammad Raziya; Ibrahim, Muhammed; Prabu, K.; Rajasankar, Srinivasagam

Background: The oxidative stress plays a crucial role in aging mediated patho-biology of neuro-degeneration and its associated behavioral impairment. The purpose of this study is to evaluate the efficacy of Withaferin-A (WA) on aging induced oxidative stress in striatum (ST) and substantia nigra (SN) of aged rat brain. Materials and Methods: Animals were randomly separated into group I – young (3 months), group II – aged (24 months), group III – aged rat supplemented with WA (50mg/kg b.w once in a day for 30 days), and group IV – young rat supplemented with WA (50mg/kg b.w). After 30 days, through cervical dislocation, animals were sacrificed and the striatum and substantia nigra (mid brain) were dissected out from brain. Brain tissues were subjected to the estimation of enzymic antioxidants, levels of free radical production and apoptotic analyses (active caspase-3 and DAPI staining). Results: The data demonstrated reduced motor activity (p<0.001), increased Reactive oxygen species (ROS) (p<0.001), reduced enzymic antioxidant defense system (p<0.001), increased active caspase-3 activity (p<0.001) and increased apoptotic nuclear morphology (p<0.001) in aged ST and SN compared to young, which was significantly reversed by the supplementation of WA. Conclusion: The evidence from the present data emphasizes that WA bears beneficial property in the protection of striatal and nigral neuron from aging mediated oxidative stress and its associated apoptosis.

WESTERN Australia; SUBSTANTIA nigra; OXIDATIVE stress; AGING; ACTIVE aging; RATS; ALBINISM

Drug Invention Today, 2019, Vol 12, Issue 3, p425

0975-7619

Academic Journal