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- Title
In vitro evaluation of the inhibitory effect of lutein from marigold flower on sorbitol accumulation.
- Authors
Balaji, V.; Roy, Anitha; Lakshmi, T.
- Abstract
Introduction: In diabetic state, the slow and steady activation of sorbitol pathway results in augmented accumulation of intracellular sorbitol which ultimately ends in diabetic complications. By preventing the intracellular accumulation of sorbitol, diabetic-related complications can be averted. The aim of the present study was to evaluate the effect of lutein from marigold flowers on sorbitol accumulation. Materials and Methods: Sorbitol accumulation in the presence of lutein was evaluated by fluorescent spectrophotometry. Heparinized rat blood was used for the study. 200 μl of washed erythrocytes was added to 1 ml of Hank's balanced salt solution containing various concentrations of lutein or ascorbic acid (5-100 μg/ml). The reaction mixture contained the appropriate protein-free supernatant, 50 mM glycine buffer, 0.2 mM NAD+, and 1.28 units of sorbitol dehydrogenase. The mixture was incubated at 37°C for 30 min, and the relative fluorescence due to NADH was measured by a fluorescence spectrometer at an excitation wavelength of 366 nm and an emission wavelength of 452 nm. The experiments were performed in triplicates. Results: In the present study, lutein inhibited the sorbitol accumulation with maximum percentage inhibition of 81.58 ± 0.51% at 100 μg/ml. Standard ascorbic acid showed maximum percentage of 84 ± 0.68% at similar concentration. The half maximal inhibitory concentration value of lutein against sorbitol accumulation was found to be 38.39 μg/ml and that of the ascorbic acid was 37.37 μg/ml. Conclusion: Prevention of intracellular sorbitol accumulation is a widely recommended concept to avert diabetes-related complications. Hence, lutein may be used judiciously for the control of diabetic complications.
- Subjects
LUTEIN; SORBITOL; PEOPLE with diabetes; MARIGOLDS; SPECTROPHOTOMETRY
- Publication
Drug Invention Today, 2018, Vol 10, p2809
- ISSN
0975-7619
- Publication type
Academic Journal