In vitro evaluation of anti-biofilm activity of Orange peel extract on Streptococcus mutans.

Vignesh, S.; Geetha, R. V.

Introduction: Biofilm inhibition is considered as major drug target for the treatment of various bacterial and fungal infections, and so the pharmacological development of drugs is now extensively studied in all the parts of the world. Citrus sinensis is the widely grown crop which is easily available all over the world. The various medicinal properties of the orange peel extract such as action against colic, upset stomach, cancer, diuretic, cormunative, immune-enhancing, and skin has been listed. It can also be used to treat and prevent vitamin deficiencies, colds, flu, and scurvy and also contain antibacterial and antiviral activities. The aim of the present study is to evaluate the in vitro anti-biofilm activity of orange peel extract on Streptococcus mutants. Materials and Methods: The S. mutans which are obtained from the saliva samples are been treated with orange peel extract which is been commercially obtained from the markets. The inhibitory action of orange peel extract on biofilm formation by S. mutans was quantified by in vitro study using microtiter plate assay. Results: After incubation, the results are recorded spectrophotometrically, the extract showed 85.49% activity at concentration of 100 μg/ml. Conclusion: The inhibition activity of the orange extract on the S. mutans was significantly high. Hence, we can suggest that use of higher concentration of the orange extract will totally inhibit the activity of the S. mutans.

BIOFILMS; ORANGE peel; FRUIT extracts; STREPTOCOCCUS mutans; ANTIFUNGAL agents; DENTAL caries

Drug Invention Today, 2018, Vol 10, Issue 12, p2485

0975-7619

Academic Journal