Title

Association of Methylation and Polymorphism of Interleukin-6 Promoter Gene With Development of Rheumatoid Arthritis.

Authors

Gomes-Silva, Isaura Isabelle Fonseca; Rushansky, Eliezer; Carvalho-Neto, Francisco Geraldo; Batista Vieira, Amanda Virginia; de Araujo Mariano, Maria Helena Queiroz; de Souza, Paulo Roberto Eleutério; Alejandro Montes, Martिn; Diniz Maia, Maria Mascena

Abstract

Rheumatoid Arthritis (RA) is a complex disease with higher level of IL-6. In order to elucidate the alterations related to IL-6 gene in RA, we evaluated the -174 G/C IL-6 gene polymorphism and methylation pattern of its promoter. A total of 120 RA patients and 120 healthy controls were recruited for polymorphism analysis, and 30 individuals of both groups were randomly selected for methylation analysis. The IL-6 gene polymorphism was analyzed by PCR-RFLP and methylation pattern was analyzed by MSPPCR. Regarding IL-6 gene polymorphism, no significant difference was found between RA patients and controls (P>0.05). For DNA, two CpG regions of IL-6 promoter gene was analyzed. The first region (-1069, -1061, -1057 and -1001 CpG) did not show significant differences, whereas the second region (-628, -610, -574 and -491 CpG) showed a significant hypermethylated status (P=0.0004) in RA patients as compared with controls.These results, suggest a possible relationship between methylation pattern and the susceptibility to RA in our studied population.

Subjects

DNA methylation; GENETIC polymorphisms; INTERLEUKIN-6; RHEUMATOID arthritis; CYTOKINES

Publication

Acta Medica Iranica, 2018, Vol 56, Issue 7, p429

ISSN

0044-6025

Publication type

Academic Journal