Extraction Process Optimization and Moisturizing Performance of Fucoidan from Sargassum fusiforme.

Bingyue LIU; Xue LIU; Fengjuan WU; Guangqun CAO; Chen ZHANG; Jie YANG

[Objectives] The research aimed to optimize extraction process of fucoidan from Sargassum fusiforme (FSF) and study its moisturizing performance. [Methods] Extracting condition of FSF by cellulase hydrolysis-ultrasonic assisted extraction method was optimized. The influences of solvent pH, enzyme dosage, extraction temperature, cellulose hydrolysis time, ultrasonication time, and the ratio of material to liquid on FSF were investigated by single factor and orthogonal experiments. [Results] The optimum extraction conditions were as followings; pH, 4.5; enzyme dosage, 1 %; extraction temperature, 40°C; cellulose hydrolysis time, 2 h; ultrasonic time, 15 min; and the ratio of material to liquid, 1:10 (g: mL). Under the optimal condition, the extraction yield of FSF was 8.50%, RSD = 2.74%. The short-time hygroscopicity (within 8 h) of crude extract of fucoidan from 5. fusiforme (CEFSF) was better than glycerin, butanediol, and sodium alginate, and the moisture retention capacity of 1% CEFSF aqueous solution was better than 1% butanediol or 1% sodium alginate, and was equal to 5% glycerin under relative humidity of 43% and 81%. The determination results of skin moisture content and transepidermal water loss rate (TEWL) showed that; 5% CEFSF solution had good moisturizing effect. [Conclusions] The research could provide certain reference for deep development of S. fusiforme.

SARGASSUM; EXTRACTION (Chemistry); PLANT moisture measurement; HYDROLYSIS; SODIUM alginate

Medicinal Plant, 2018, Vol 9, Issue 3, p21

2152-3924

Academic Journal

10.19600/j.cnki.issn2152-3924.2018.03.006