Title

DEVELOPMENT AND VALIDATION OF ANALYTICAL HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF AMOXICILLIN AND PROBENCIDE IN TABLET.

Authors

Shah, Dhwani; Vegad, Kunjal; Patel, Ekta; Prajapati, Jigna; Kaushik Patel; Patel, Yogesh

Abstract

A simple, accurate, rapid, economical RP-HPLC method have been developed and validated for estimation of Amoxicillin and Probencide in combined tablet dosage form and can be used in routine analysis. In RP-HPLC, method was carried out by isocratic technique on a reversedphase: by C18 column (250×4.6 mm, 5 μm Particle Size) column kept at ambient temperature and with mobile phase containing a mixture of buffer (pH4.0)-methanol (40:60% v/v) at a flow rate of 1.0 ml/min. The average retention times for Amoxicillin and Probencide were 3.447min and 6.193min respectively. The calibration curves were linear in the concentration range of 5-15μg/ml for Amoxicillin and of 5-15μg/ml for Probencide. Correlation coefficients were found to be 0.9976 and 0.9985 for Amoxicillinand Probencide respectively. Mean assay was found to be 99.95% and 102.73% for Amoxicillin and Probencide respectively. All the three methods were compared statistically using ANOVA and it was found that they are not significantly differ indicating their suitability in routine analysis.

Subjects

HIGH performance liquid chromatography; AMOXICILLIN; PROBENECID (Drug); DRUG tablets; DOSAGE forms of drugs; ESTIMATION theory

Publication

Pharma Science Monitor, 2017, Vol 8, Issue 3, p108

ISSN

0976-9242

Publication type

Academic Journal