EVALUATION OF ANTI CATARACT ACTIVITY OF ISOLATED FRACTIONS OF ALBIZIA PROCERA STEM BARK CHLOROFORM EXTRACT: TARGETING NA K ATPASE IN GOAT LENSES.

KumarP., Praveen; A., Ramesh; K., Prasad; K., Gnananath

The present study was investigate possible mechanism of action of anti cataract activity of fractions isolated from Albizia procera stem bark chloroform extract against glucose induced cataract lenses. Goat eye lenses were divided into nine groups. Group I lenses were incubated in artificial aqueous humor with glucose concentration 5.5 mM (normal control). Group II lenses were incubated with glucose concentration 55 mM (toxic control), remain groups were incubated with glucose concentration 55 mM along with isolated fractions (4mg/ml). After 72 hrs of incubation, lens was subjected to photographic evaluation for opacity, lens homozenate supernatant used for estimation of total proteins, glutathione and Na K ATPase activity. After 72 h of incubation in glucose 55 mM, lens becomes completely opaque as against lenses incubated in 5.5 mM glucose. Incubated lenses with isolated fractions C of Albizia procera stem bark chloroform extract at concentration 4 mg/ml, seems to greater retard the progression of lens opacification, increase total protein (P<0.05) and glutathione (P<0.05) and balanced Na and K levels by improving Na K ATPase activity (P<0.05) in cataract lens compare to other fractions treated lenses. It was concluded that fraction C of Albizia procera stem bark chloroform extract possesses anti cataract potential due to their anti oxidant activity and improving Na K ATPase activity.

OPHTHALMIC lenses; TALL albizia; CHLOROFORM; CATARACT; GLUCOSE; OPACITY (Optics); GLUTATHIONE; ANTIOXIDANTS

Advances in Pharmacology & Toxicology, 2017, Vol 18, Issue 1, p1

0973-2381

Academic Journal