Title

DEVELOPMENT OF HPTLC METHOD FOR ESTIMATION OF EMBELIN IN VIDANGA (EMBELIA TSJERIAM-COTTAM) AND DETERMINATION OF ITS ANTIBACTERIAL ACTIVITY.

Authors

Kulkarni, Sweta

Abstract

Vidanga (Embelia tsjeriam-cottam) is a promising medicinal plant of high medicinal value documented in Ayurvedic classics and traditional Indian medicines. It is said to be the best medicine against human pathogens. Embelin (2,5-dihydroxy-3-undecyl-2,5-cyclohexadiene-1,4-benzoquinone) is a phenolic compound found in the fruits of Embelia tsjeriamcottam and is responsible for the medicinal properties of the plant. A new simple, accurate, precise High Performance Thin Layer Chromatographic (HPTLC) method was developed and validated for determination of Embelin. The method employed aluminium precoated silica gel 60 F254 using Toluene: Ethyl Acetate:Formic acid (6:3.5:0.5 v/v) as mobile phase. Detection wavelength chosen was 291nm. The Rf value of Embelin was found to be 0.59 ± 0.02. The calibration curve was found to be linear over a range of 200 - 1000 ng/ band. Developed HPTLC method showed good regression (r² = 0.999 ± 0.0020). The % recovery was found to be 99.4-101.6%. The method showed a good percentage purity of the formulation. This method can be used for quantification of Embelin in marketed formulation. The antibacterial activity of Embelin and ethanolic extracts of this plant was determined against gram-positive bacterial strain (Staphylococcus aureus) and gram-negative bacterial strains (Escherichia coli).

Subjects

INDIA; DRUG development; BLACK pepper (Plant); ANTIBACTERIAL agents; MEDICINAL plants; MEDICINE; AYURVEDIC medicine; HIGH performance liquid chromatography; THERAPEUTICS

Publication

International Journal of Pharmacy & Life Sciences, 2016, Vol 7, Issue 9, p44

ISSN

0976-7126

Publication type

Academic Journal