Title

Isolation of flavonoids from Delonix data and determination of its rutin content using capillary electrophoresis.

Authors

Al-Taweel, Areej Mohammed; Abdel-Kader, Maged Saad; Fawzy, Ghada Ahmed; Perveen, Shagufta; Maher, Hadir Mohamed; Al-Zoman, Nourah Zoman; Al-Shehri, Mona Mohamed; Al-Johar, Haya; AI-Showiman, Hessa

Abstract

Delonix elata (L.) Gamble (Fabaceae) is an important, traditionally used plant in Saudi Arabia. It is used to relieve rheumatic pain, flatulence and the seeds are employed as purgatives. The aim of the present study was to isolate chemical constituents of the w-butanol fraction (BF) of D. elata and to find out, by capillary electrophoresis (CE), percentage of rutin present in this BF. Three quercetin glycosides and one kaempferol rutinoside were isolated from the BF of aerial parts of D. elata; namely, Quercetin 3-0-rutinoside-7-0-glucoside (1), Quercetin 3,7-diglucoside (2), Quercetin 3-O-rutinoside (RUT) (3) and Kaempferol 3-O-rutinoside (4). Rutin, an active constituent has been reported to possess good pharmacological as well as therapeutic potentials. A sensitive and rapid procedure for quantitative determination of RUT by capillary electrophoresis was developed and its content was found to be 7.349 mg/gm, relative to «-butanol fraction and 18.373 mg%, relative to the dry powder of D. elata. The method could be recommended for approval and use in the pharmaceutical and food industries.

Subjects

SAUDI Arabia; FLAVONOIDS; DELONIX; CAPILLARY electrophoresis; RUTIN; BUTANOL

Publication

Pakistan Journal of Pharmaceutical Sciences, 2015, Vol 28, p1897

ISSN

1011-601X

Publication type

Academic Journal