Detection of Chlorogenic Acid, Macranthoidin B and Dipsacoside B in FLOS LONICERAE Grown in Guizhou Province by UPLC-PDA-ELSD.

Liucun ZHAO; Guanqing WEN; Li LI; Shuaihui ZHANG; Shiyun YE

[Objective] To detect chlorogenic acid, macranthoidin B and dipsaocoside B in FLOS LONICERAE grown in Guizhou Province by UPLC-PDA-ELSD, and to investigate the quality of FLOS LONICERAE grown in Guizhou Province. [Methods] ACQUTTYUPLC BEH C18 chromatographic column (2.1 mm × 100 mm, 1.7 μm) was adopted; mobile phase was acetonitrile (A) - 0.4% acetic acid water (B); gradient elution was as follows: 0-2 min, 7% A; 2-2.3 min. 7%-28% A; 2.3-10 min, 28% A; 10-10.5 min, 28%-7% A; gradient flow rate was as follows; 0-2 min, 0.35 mL/min; 2-2.3 min, 0.35-0.25 mL/min; 2.3-2.6 min, 0.25-0.15 mL/min; 2.6-2.8 min, 0.15-0.1 mL/min; 2.8-4 min, 0.1-0.2 mL/min; 4-4.3 min, 0.2-0.3 mL/min; 4.3-4.5 min, 0.3-0.35 mL/min; 4.5-10.5 min, 0. 35 mL/min; PDA (ultraviolet detector) was used to detect the ehlorogenie acid (330 nm wavelength); ELSD (evaporative light-scattering detector) was used to detected the macranthoidin B and dipsacoside B; temperature of the drift tube was 60 °C ; sprayer was 48 °C; gas pressure was 137.9 kPa; and column temperature was 50 °C. [Results] Chlorogenie acid, macranthoidin B and dipsaooside B showed good linear relationship within the ranges of 0.3045-1.5225 μg/mL (R2 = 0.9994), 0.449-1.4368 μg/mL (R2 = 0.9993) and 0.0446-0.223 μg/mL (R2= 0.9994), respectively. [Conclusions] This method could accurately detect the contents of chlorogenie acid, macranthoidin B and dipsaeoside B in FLOS LONIGERAE grown in Guizhou Province, effectively shorten the measurement time and save reagents, so that it was suitable to detect the quality of FLOS LONICERAE grown in Guizhou Province.

HONEYSUCKLES; PHYTOTHERAPY; CHLOROGENIC acid; THERAPEUTIC use of plant extracts; ACETIC acid; THERAPEUTICS

Medicinal Plant, 2015, Vol 6, Issue 7/8, p17

2152-3924

Academic Journal