Effects of exogenous substances on saponin content in root of Polygala tenuifolia Willd.

TENG Hong-mei; XIE Xu-rong; WANG Ling-li; HU Zheng-hai

[Objective] The effects of exogenous substances on the saponin content in root of Polygala tenuifolia Willd were studied to provide references for improving the medicinal ingredients of planting of Polygala tenuifolia Willd. [Method] During the leaf-expansion period, naphthalene acetic acid (25,50,100 mg/L), gibberellin (50, 150, 300 mg/L), cytokinin (100, 200, 300 mg/L), abscisic acid (5, 10, 15 mg/L), methyl jasmonate (200, 300, 400 jxmol/L) and salicylic acid (50, 100, 200 mg/L) were sprayed respectively on the leaf surface, and the effects of the different concentrations of exogenous substances on saponin content in root of the field-planting Polygala tenuifolia Willd were compared. [ Result ] The low, medium, and high concentrations of four kinds of exogenous hormone, naphthalene acetic acid, gibberellin, cytokinin and abscisic acid could enhance the saponins in root of Polygala tenuifolia Willd to 4.07, 3.98, 3.91 times; 22.33, 14.21, 13.72 times; 7.8, 6.2, 5.4 times; 2.1, 2.3, 2.6 times respectively, compared to the control. Low, medium, and high concentrations of two kinds of elicitors of methyl jasmonate and salicylic acid could enhance the content of saponin to 6.6, 14.2, 7.6 times and 1.65, 2.95, 3.49 times respectively, compared to the control. The effecting order of 6 kinds of exogenous substances on the saponins accumulation was gibberellin > methyl jasmonate > cytokinin > naphthalene acetic acid > salicylic acid > abscisic acid. [Conclusion] Exogenous substances could significantly improve the saponin content in root of Polygala tenuifolia Willd by spraying on leaf surface, and this method could be applied in production practice.

Journal of Southern Agriculture, 2014, Vol 45, Issue 12, p2143

2095-1191

Academic Journal

10.3969/j:issn.2095-1191.2014.12.2103