Title

Screening for potential α-Glucosidase inhibitors in Coptis chinensis franch extract using ultrafiltration LC-ESI-MS.

Authors

Hui Zhou; Tao Jiang; Zhengyang Wang; Shen Ren; Xi Zhao; Wei Wus; Lianhai Jiang; Zhiqiang Liu; Lirong Teng

Abstract

Naturally existing α-glucosidase inhibitors from traditional herbal medicines have attracted considerable interest to treat type 2 diabetes mellitus (T2DM). Hundreds of herbs have been reported to have the potential to inhibit α-glucosidase. However, most common methods to examine the inhibitors of α-glucosidase are usually time-consuming. In the current study, the screening of α-glucosidase ligands from Coptis chinensis Franch extract was undertaken by ultrafiltration liquid chromatography coupled to electrospray ionization tandem mass spectrometry (ultrafiltration LC- ESI-MSn). Resultantly, the enzyme inhibition studies showed that Coptis chinensis Franch extract carries the strongest α-glucosidase inhibitory activity among the five kinds of Chinese herbal extracts. Subsequently, five compounds that could bind to α-glucosidase in the Coptis chinensis Franch extract were found using ultrafiltration liquid chromatography, and their structures were identified by ESI-MSn to be coptisine, epiberberine, jatrorrhizine, berberine, palmatine. Cumulatively, these results were anticipated to be encouraging for applying the Coptis chinensis Franch extracts as efficient anti-diabetic drug candidates.

Subjects

MEDICAL screening; GLUCOSIDASE inhibitors; COPTIS chinensis; EXTRACTION (Chemistry); ULTRAFILTRATION; HERBAL medicine

Publication

Pakistan Journal of Pharmaceutical Sciences, 2014, Vol 27, p2007

ISSN

1011-601X

Publication type

Academic Journal