Works matching Cloning

Results: 5000

Restriction site free cloning (RSFC) plasmid family for seamless, sequence independent cloning in Pichia pastoris.

Published in:

Microbial Cell Factories, 2015, v. 14, n. 1, p. 1, doi. 10.1186/s12934-015-0293-6

By:

Vogl, Thomas;
Ahmad, Mudassar;
Krainer, Florian W.;
Schwab, Helmut;
Glieder, Anton

Publication type:

Article

Analysis of an epigenetic argument against human reproductive cloning.

Published in:

Reproductive BioMedicine Online (Reproductive Healthcare Limited), 2006, v. 13, n. 2, p. 278, doi. 10.1016/S1472-6483(10)60627-X

By:

Nordgren, Anders

Publication type:

Article

الإستنساخ أنواعه وأحكامه.

Published in:

Rihan Journal for Science Publishing, 2022, n. 23, p. 308

By:

شمس الاسلام شمس صافی

Publication type:

Article

Cloning and sequencing analysis of whole Spiroplasma genome in yeast.

Published in:

Frontiers in Microbiology, 2024, p. 1, doi. 10.3389/fmicb.2024.1411609

By:

Masaki Mizutani;
Sawako Omori;
Noriko Yamane;
Yo Suzuki;
Glass, John I.;
Ray-Yuan Chuang;
Takema Fukatsu;
Shigeyuki Kakizawa

Publication type:

Article

TA-GC cloning: A new simple and versatile technique for the directional cloning of PCR products for recombinant protein expression.

Published in:

PLoS ONE, 2017, v. 12, n. 11, p. 1, doi. 10.1371/journal.pone.0186568

By:

Niarchos, Athanasios;
Siora, Anastasia;
Konstantinou, Evangelia;
Kalampoki, Vasiliki;
Lagoumintzis, George;
Poulas, Konstantinos

Publication type:

Article

Towards Practical Conservation Cloning: Understanding the Dichotomy Between the Histories of Commercial and Conservation Cloning.

Published in:

Animals (2076-2615), 2025, v. 15, n. 7, p. 989, doi. 10.3390/ani15070989

By:

Novak, Ben J.;
Brand, Stewart;
Phelan, Ryan;
Plichta, Sasha;
Ryder, Oliver A.;
Wiese, Robert J.

Publication type:

Article

IRDL Cloning: A One-Tube, Zero-Background, Easy-to-Use, Directional Cloning Method Improves Throughput in Recombinant DNA Preparation.

Published in:

PLoS ONE, 2014, v. 9, n. 9, p. 1, doi. 10.1371/journal.pone.0107907

By:

Wang, Jiancai;
Xu, Ronghua;
Liu, Aizhong

Publication type:

Article

Homologous alignment cloning: a rapid, flexible and highly efficient general molecular cloning method.

Published in:

PeerJ, 2018, p. 1, doi. 10.7717/peerj.5146

By:

Tan, Lendl;
Strong, Emily J.;
Woods, Kyra;
West, Nicholas P.

Publication type:

Article

A strategy for in-house production of a positive selection cloning vector from the commercial pJET1.2/blunt cloning vector at minimal cost.

Published in:

3 Biotech, 2022, v. 12, n. 9, p. 1, doi. 10.1007/s13205-022-03289-x

By:

Nawawi, Omar;
Abdullah, Mohd Puad;
Yusuf, Chong Yu Lok

Publication type:

Article

In- & Out-Cloning: plasmid toolboxes for scarless transcription unit and modular Golden Gate acceptor plasmid assembly.

Published in:

Synthetic Biology (23977000), 2024, v. 9, n. 1, p. 1, doi. 10.1093/synbio/ysae016

By:

Vries, Stijn T de;
Köbel, Tania S;
Sanal, Ahmet;
Schindler, Daniel

Publication type:

Article

Efficient cloning of linear DNA inserts (ECOLI) into plasmids using site-directed mutagenesis.

Published in:

Scientific Reports, 2024, v. 14, n. 1, p. 1, doi. 10.1038/s41598-024-72169-6

By:

Paclikova, Petra;
Harnos, Jakub

Publication type:

Article

A series of novel directional cloning and expression vectors for blunt-end ligation of PCR products.

Published in:

Biotechnology Letters, 2010, v. 32, n. 3, p. 439, doi. 10.1007/s10529-009-0166-3

By:

Qiong Wu;
Xing Zhong;
Chao Zhai;
Ju Yang;
Xiaomei Chen;
Liang Chen;
Wenfeng Wang;
Lixin Ma

Publication type:

Article

A simple and rapid strategy for the molecular cloning and monitoring of mouse HtrA2 serine protease.

Published in:

Biotechnology Letters, 2008, v. 30, n. 3, p. 397, doi. 10.1007/s10529-007-9556-6

By:

Goo-Young Kim;
Min-Kyung Nam;
Sang-Soo Kim;
Ho-Young Kim;
Sang-Kyu Lee;
Rhim, Hyangshuk

Publication type:

Article

Escherichia coli BW25113 Competent Cells Prepared Using a Simple Chemical Method Have Unmatched Transformation and Cloning Efficiencies.

Published in:

Frontiers in Microbiology, 2022, v. 13, p. 1, doi. 10.3389/fmicb.2022.838698

By:

Yang, Yuqing;
Yu, Qiaoli;
Wang, Min;
Zhao, Rui;
Liu, Huaiwei;
Xun, Luying;
Xia, Yongzhen

Publication type:

Article

pELMO, an optimised in-house cloning vector.

Published in:

AMB Express, 2017, v. 7, n. 1, p. 1, doi. 10.1186/s13568-017-0324-2

By:

Ramos, Andrea;
Muñoz, Marina;
Moreno-Pérez, Darwin;
Patarroyo, Manuel

Publication type:

Article

An efficient method for integration of PCR fragments into adjacent or overlapping restriction sites during gene cloning.

Published in:

3 Biotech, 2018, v. 8, n. 4, p. 1, doi. 10.1007/s13205-018-1214-2

By:

Makam, Shivakiran;
Srirama, Krupanidhi;
Dirisala, Vijaya Ramu;
Reddy, Prakash Narayana

Publication type:

Article

Insights into the roles of sperm in animal cloning.

Published in:

Stem Cell Research & Therapy, 2020, v. 11, n. 1, p. 1, doi. 10.1186/s13287-020-01599-6

By:

Qu, Pengxiang;
Wang, Yongsheng;
Zhang, Chengsheng;
Liu, Enqi

Publication type:

Article

Human embryos cloned for stem cells: work not seen as leap toward reproductive cloning.

Published in:

2004

By:

Vastag, Brian

Publication type:

journal article

UN Delays Cloning Decision.

Published in:

JAMA: Journal of the American Medical Association, 2003, v. 290, n. 23, p. 3057, doi. 10.1001/jama.290.23.3057-d

By:

Stephenson, Joan

Publication type:

Article

Cloning in action: can embryo splitting, induced pluripotency and somatic cell nuclear transfer contribute to endangered species conservation?

Published in:

Biological Reviews, 2023, v. 98, n. 4, p. 1225, doi. 10.1111/brv.12951

By:

Swegen, Aleona;
Appeltant, Ruth;
Williams, Suzannah A

Publication type:

Article

A high-throughput, restriction-free cloning and screening strategy based on ccdB-gene replacement.

Published in:

Microbial Cell Factories, 2014, v. 13, n. 1, p. 1, doi. 10.1186/1475-2859-13-38

By:

Lund, Bjarte Aarmo;
Schrøder Leiros, Hanna-Kirsti;
Kjæreng Bjerga, Gro Elin

Publication type:

Article

Development of pENTR-NeCo-lacZα vectors for the preparation of negative control constructs in Gateway cloning.

Published in:

Bioscience, Biotechnology & Biochemistry, 2024, v. 88, n. 7, p. 784, doi. 10.1093/bbb/zbae061

By:

Kuzuhara, Taiki;
Monden, Kota;
Hachiya, Takushi;
Nakagawa, Tsuyoshi

Publication type:

Article

A streamlined strategy for self-production of a commercial positive selection vector, the pJET1.2/blunt cloning vector, using common laboratory E. coli strains.

Published in:

3 Biotech, 2023, v. 13, n. 7, p. 1, doi. 10.1007/s13205-023-03647-3

By:

Nawawi, Omar;
Abdullah, Mohd Puad;
Yusuf, Chong Yu Lok

Publication type:

Article

Cloning of Clostridium perfringens iota toxin gene in Escherichia coli.

Published in:

Archives of Razi Institute, 2018, v. 73, n. 2, p. 107

By:

Seyed Sayyah, P.;
Golestani Eimani, B.;
Pilehchian Langroudi, R.

Publication type:

Article

Hybrid Quantum Cloning Machine.

Published in:

Quantum Information Processing, 2007, v. 6, n. 4, p. 197, doi. 10.1007/s11128-007-0053-6

By:

Adhikari, S.;
Pati, A.;
Chakrabarty, I.;
Choudhury, B.

Publication type:

Article

An efficient method for targeted cloning of large DNA fragments from Streptomyces.

Published in:

Applied Microbiology & Biotechnology, 2023, v. 107, n. 18, p. 5749, doi. 10.1007/s00253-023-12685-z

By:

Tian, Yuqing;
Li, Dong;
Wang, Kai;
Wei, Baoting;
Zhang, Jihui;
Li, Jine

Publication type:

Article

Gene cloning and seamless site-directed mutagenesis using single-strand annealing (SSA).

Published in:

Applied Microbiology & Biotechnology, 2018, v. 102, n. 23, p. 10119, doi. 10.1007/s00253-018-9348-z

By:

Luo, Zhixin;
Wang, Shanhe;
Jiao, Beilei;
Yuan, Dan;
Dai, Dongmei;
Wang, Lixian;
Xu, Kun;
Wang, Xin

Publication type:

Article

Clonación de mamíferos: regulación y participación pública en Argentina y Reino Unido.

Published in:

Revista Iberoamericana de Ciencia, Tecnologia y Sociedad, 2020, v. 15, n. 44, p. 43

By:

Bilañski, Gisele

Publication type:

Article

A MultiSite Gateway Toolkit for Rapid Cloning of Vertebrate Expression Constructs with Diverse Research Applications.

Published in:

PLoS ONE, 2016, v. 11, n. 8, p. 1, doi. 10.1371/journal.pone.0159277

By:

Fowler, Daniel K.;
Stewart, Scott;
Seredick, Steve;
Eisen, Judith S.;
Stankunas, Kryn;
Washbourne, Philip

Publication type:

Article

Rapid and Robust PCR-Based All-Recombinant Cloning Methodology.

Published in:

PLoS ONE, 2016, v. 11, n. 3, p. 1, doi. 10.1371/journal.pone.0152106

By:

Dubey, Abhishek Anil;
Singh, Manika Indrajit;
Jain, Vikas

Publication type:

Article

A ligation and restriction enzyme independent cloning technique: an alternative to conventional methods for cloning hard-to-clone gene segments in the influenza reverse genetics system.

Published in:

Virology Journal, 2020, v. 17, n. 1, p. 1, doi. 10.1186/s12985-020-01358-2

By:

Bhat, Sushant;
Bialy, Dagmara;
Sealy, Joshua E.;
Sadeyen, Jean-Remy;
Chang, Pengxiang;
Iqbal, Munir

Publication type:

Article

MCT cloning: a seamless cloning strategy for inserting DNA fragments.

Published in:

Biotechnology & Biotechnological Equipment, 2018, v. 32, n. 5, p. 1298, doi. 10.1080/13102818.2018.1507756

By:

Li, Wen-Ying;
Liu, Chang-Jun;
Wu, Lei;
Wu, Jin-Feng;
Yin, Xi-Nong;
Deng, Kai-Hang;
Zhang, Dong-Yi;
Meng, Er

Publication type:

Article

Insert restriction enzyme cutting-free cloning strategy for expression plasmid construction.

Published in:

Biotechnology & Biotechnological Equipment, 2017, v. 31, n. 5, p. 1033, doi. 10.1080/13102818.2017.1351310

By:

Lou, Ye-jiang;
Jin, Jie

Publication type:

Article

NONLOCAL CLONING FOR A STATE NEAR A GIVEN ONE WITH SINGLE-PHOTON INTERFERENCE.

Published in:

International Journal of Quantum Information, 2009, v. 7, n. 7, p. 1331, doi. 10.1142/S0219749909005663

By:

YANG SHAO;
YAN-HUI ZHOU;
AI-DONG ZHU

Publication type:

Article

The Ethical-Theological and Legal Approaches to Cloning.

Published in:

Nosileftiki, 2010, v. 49, n. 4, p. 338

By:

Katsimigas, George;
Kaba, Evridiki

Publication type:

Article

Cloning and expression of selected ABC transporters from the Arabidopsis thaliana ABCG family in Pichia pastoris.

Published in:

PLoS ONE, 2019, v. 14, n. 1, p. 1, doi. 10.1371/journal.pone.0211156

By:

Gräfe, Katharina;
Shanmugarajah, Kalpana;
Zobel, Thomas;
Weidtkamp-Peters, Stefanie;
Kleinschrodt, Diana;
Smits, Sander H. J.;
Schmitt, Lutz

Publication type:

Article

Quantum Superimposing Multiple Anti-Cloning Machine.

Published in:

International Journal of Theoretical Physics, 2007, v. 46, n. 10, p. 2599, doi. 10.1007/s10773-007-9374-6

By:

Yan-ling Li;
Jian Feng;
Bao-long Liang;
Ya-fei Yu

Publication type:

Article

Pyrite cloning: a single tube and programmed reaction cloning with restriction enzymes.

Published in:

Plant Methods, 2018, v. 14, n. 1, p. N.PAG, doi. 10.1186/s13007-018-0359-7

By:

Fischer, Matthew D.;
Mgboji, Emmanuel;
Liu, Zhongchi

Publication type:

Article

The plasmid vectors, pBS2ndd and pBS3ndd, for versatile cloning with low background in Escherichia coli.

Published in:

World Journal of Microbiology & Biotechnology, 2018, v. 34, n. 6, p. 1, doi. 10.1007/s11274-018-2466-z

By:

Rotchanapreeda, Tiwa;
Ngonsawan, Wasinee;
Klomtun, Manadsaree;
Somana, Jamorn

Publication type:

Article

A new method to customize protein expression vectors for fast, efficient and background free parallel cloning.

Published in:

BMC Biotechnology, 2013, v. 12, n. 1, p. 1, doi. 10.1186/1472-6750-13-12

By:

Scholz, Judith;
Besir, Hüseyin;
Strasser, Claudia;
Suppmann, Sabine

Publication type:

Article

HUMAN REPRODUCTIVE CLONING, EMBRYO STEM CELLS AND GERMLINE GENE INTERVENTION: AN ISRAELI PERSPECTIVE.

Published in:

Medicine & Law (World Association for Medical Law), 2003, v. 22, n. 4, p. 701

By:

Revel, Michel

Publication type:

Article

An Improved Homologous Recombination Method for Rapid Cloning of the Antibody Heavy Chain Gene and Its Comparison with the Homologous Recombination and Traditional Cloning Methods.

Published in:

Novelty in Biomedicine, 2015, n. 4, p. 171

By:

Hajirezaei, Masoumeh;
Darbouy, Mojtaba;
Rasouli, Manoochehr;
Kazemi, Bahram

Publication type:

Article

Peripheral infrastructure vectors and an extended set of plant parts for the Modular Cloning system.

Published in:

PLoS ONE, 2018, v. 13, n. 5, p. 1, doi. 10.1371/journal.pone.0197185

By:

Gantner, Johannes;
Ordon, Jana;
Ilse, Theresa;
Kretschmer, Carola;
Gruetzner, Ramona;
Löfke, Christian;
Dagdas, Yasin;
Bürstenbinder, Katharina;
Marillonnet, Sylvestre;
Stuttmann, Johannes

Publication type:

Article

In vivo cloning of up to 16 kb plasmids in E. coli is as simple as PCR.

Published in:

PLoS ONE, 2017, v. 12, n. 8, p. 1, doi. 10.1371/journal.pone.0183974

By:

Huang, Faqing;
Spangler, Joseph Rankin;
Huang, Allen Yang

Publication type:

Article

Cloning and Sequencing of cDNA and Genomic DNA Encoding PDM Phosphatase of Fusarium moniliforme.

Published in:

Journal of Biochemistry, 2006, v. 140, n. 6, p. 813, doi. 10.1093/jb/mvj215

By:

Yoshida, Hiroshi;
Iizuka, Mari;
Narita, Takao;
Norioka, Naoko;
Norioka, Shigemi

Publication type:

Article

Microcavity-assisted cloning (MAC) of hard-to-clone HepG2 cell lines: cloning made easy.

Published in:

BMC Biotechnology, 2024, v. 24, n. 1, p. 1, doi. 10.1186/s12896-024-00911-z

By:

Mlakar, Vid;
Lesne, Laurence;
Vossio, Stefania;
Dupanloup, Isabelle;
Gloor, Yvonne;
Moreau, Dimitri;
Ansari, Marc

Publication type:

Article

Chancen und Risiken der Reproduktionsbiotechnologien in der Tierzucht - Verfahren des Gene Editing und des Klonens.

Published in:

Züchtungskunde, 2024, v. 96, n. 1, p. 10

By:

HÖLKER, MICHAEL

Publication type:

Article

Advances in molecular cloning.

Published in:

Molecular Biology, 2016, v. 50, n. 1, p. 1, doi. 10.1134/S0026893316010131

By:

Ashwini, Malla;
Murugan, Shanmugaraj;
Balamurugan, Srinivasan;
Sathishkumar, Ramalingam

Publication type:

Article

Efficient cloning of plant genomes into bacterial artificial chromosome (BAC) libraries with larger and more uniform insert size.

Published in:

Plant Biotechnology Journal, 2004, v. 2, n. 3, p. 181, doi. 10.1111/j.1467-7652.2004.00065.x

By:

Chalhoub, Boulos;
Belcram, Harry;
Caboche, Michel

Publication type:

Article

Recombinant expression library of Pyrococcus furiosus constructed by high-throughput cloning: a useful tool for functional and structural genomics.

Published in:

Frontiers in Microbiology, 2015, p. 1, doi. 10.3389/fmicb.2015.00943

By:

Hui Yuan;
Li Peng;
Zhong Han;
Juan-Juan Xie;
Xi-Peng Liu

Publication type:

Article