Antiviral properties of herbal flavonoids - inhibitors of the DNA and RNA synthesis.

Palchykovska, L. G.; Vasylchenko, O. V.; Platonov, M. O.; Starosyla, D. B.; Porva, J. I.; Rymar, S. J.; Atamaniuk, V. P.; Samijlenko, S. P.; Rybalko, S. L.

Aim. To investigate influence of the Deschampsia caespitosa L. and Calamagrostis epogeios L. herbal extract (HE) and its main components - quercetin derivatives - on the DNA and RNA synthesis; to perform comparative analysis of their antiviral activities. Methods. The model transcription system of bacteriophage T7 (RNAP T7) and polymerase chain reaction (PCR) were used as test systems. For antiviral studies there were employed models of influenzal and herpetic infections and hepatitis C. Results. It was shown, that HE, its major component 7, 3'-dimetoxyquercetin (DMQ) its minor component 5,7,3',4'-tetramethoxyqeercetin tetramethoxyqeercetin (TMQ) effectively inhibited the RNA synthesis in the RNAP T7 system with the corresponding values of IC50 - 0.07, 4 and 1 μg/ml. Minimal concentrations of the agents IC90, completely inhibiting PCR, were - 8, 30 and 40 μg/ml for HE, TMQ and DMQ, accordingly. All the studied preparations revealed high multiple antiviral activities against the RNA- and DNA-containing viruses. Conclusions. Antiviral activity of the herbal extract is likely determined by the combined action of its components, TMQ and DMQ including. Bearing in mind multi-target profile of quercetin derivatives, an assumption may be made, that antiviral mechanism of the agents studied consists in blocking of enzymatic systems of the RNA and DNA synthesis.

NUCLEIC acids; RNA synthesis; DEOXYRIBOSE; QUERCETIN; FLAVONOIDS; BIOFLAVONOIDS

Biopolymers & Cell, 2013, Vol 29, Issue 2, p150

0233-7657

Academic Journal

10.7124/bc.000813