Title

INVESTIGATION OF P-CHLOROANILINE FORMATION IN THE REACTIONS BETWEEN DIFFERENT ENDODONTIC IRRIGANTS.

Authors

Bjelović, Ljiljana Z.; Glišić, Biljana Đ.; Živković, Marija D.; Kanjevac, Tatjana V.

Abstract

The aim of this study was to determine whether p-chloroaniline (PCA) is formed in the reactions of sodium hypochlorite (NaOCl) with final rinse chlorhexidine (CHX), QMix (combination of ethylenediaminetetraacetic acid (EDTA), CHX and detergent) and EDTA/CHX solutions by thin-layer chromatography (TLC), proton nuclear magnetic resonance (¹H NMR) and infrared (IR) spectroscopy. Commercially available 5.25% NaOCl solution was mixed with 2% CHX and QMix in 1:1 (v/v) ratio at room temperature. Furthermore, 2% CHX was associated with 17% EDTA under the same experimental conditions. The obtained solutions were evaluated qualitatively for color changing, precipitate and/or bubble formation and analyzed by TLC chromatography. The association products were investigated by spectroscopic (¹H NMR and IR) methods in order to determine whether they contain PCA. It was found that interactions between NaOCl/CHX and CHX/EDTA led to forming of brown and white precipitate. When NaOCl was mixed with QMix, an orange-brown precipitate was formed. PCA was not detected as a product of the reactions between NaOCl and final rinse solutions of CHX, QMix and EDTA/CHX association.

Subjects

PROTON magnetic resonance; NUCLEAR magnetic resonance; ETHYLENEDIAMINETETRAACETIC acid; THIN layer chromatography; SODIUM hypochlorite; CHROMATOGRAPHIC analysis

Publication

Kragujevac Journal of Science, 2019, Issue 41, p43

ISSN

1450-9636

Publication type

Academic Journal

DOI

10.5937/kgjsci1941043b