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- Title
Microdissection genotyping of mixed glial and primitive neuroectodermal central nervous system neoplasm.
- Authors
Mohan, Deepak; Rao, Gutti R; Swalsky, Patricia A; Bakker, Anke; Martinez, A Julio; Finkelstein, Sydney D
- Abstract
A 22-year-old man with previous radiation treatment for childhood astrocytoma underwent resection of a right parietooccipital lesion. Histopathology revealed a malignant neoplasm with areas of astrocytic and primitive neuroectodermal components. To resolve the relationship and cellular origin, representative tissue was microdissected from several targets, obtaining a balanced mixture of each element. Nonneoplastic brain parenchyma was separately microdissected to determine polymorphic marker informativeness and to serve as an internal negative control. Despite the relatively small quantity of tissue removed for each microdissection target, sufficient material was available for reliable, balanced, polymerase chain reaction-format genotyping encompassing a panel of tumor suppressor genes and genetic loci associated with these forms of neoplasia. The findings revealed distinct discordant genotypic profiles for each of the neoplastic components. The efficacy of the approach used for molecular analysis of this complex neoplasm and the implication of the genotypic findings are discussed.
- Publication
Archives of pathology & laboratory medicine, 2004, Vol 128, Issue 10, p1161
- ISSN
1543-2165
- Publication type
Journal Article
- DOI
10.5858/2004-128-1161-MGOMGA