Antioxidant effects of ellagic acid on testicular tissue of rats exposed to tobacco smoke metabolite-acetic acid.

Kaya, Nalan; Ozan, Gonca; Dabak, Durrin Ozlem; Gur, Seyfettin; Ozan, Ibrahim Enver

Aim: Tobacco smoke negatively affects the male reproductive system. Ellagic acid (EA) has protective effects against oxidative damage. The aim of the study was to examine the protective effects of EA on testis of rats exposed to tobacco smoke. Material and Methods: Twenty-four male Spraque-Dawley rats were divided into 4 groups (n=6): Control, tobacco smoke (TS), tobacco smoke corn oil (TS C) and tobacco smoke EA (TS EA). TS, TS C and TS EA groups were exposed to tobacco smoke 1 hour twice a day and EA was applied 12 mg/kg every other day. Testis tissues were removed. eNOS immunohistochemical stain and TUNEL methods were applied. Biochemical analyzes and sperm analyzes were performed. Results: Degeneration in germinative epithelium, cell debris in the seminiferous tubule lumen, seperation in basement membrane, atrophic tubules, vascular congestion and edema in interstitial area were observed in TS and TS C groups. Increased apoptotic cells and eNOS immunreactivity were observed in TS and TS C groups. EA administration caused a decrease in histological alterations, eNOS immunreactivity and apoptotic cells. Increased MDA levels, decreased CAT and GSH-Px activities were observed in TS and TS C groups. MDA levels decreased, CAT and GSH-Px activities increased in TS EA group. A significant increase in the amount of abnormal sperm was detected in TS and TS C groups. The reduction in the amount of abnormal sperm was detected in TS EA group. Conclusions: Exposure to TS led to marked alterations on testes tissue and treatment with EA might prevent these toxic effects.

ANTIOXIDANTS; TOBACCO

Journal of Turgut Ozal Medical Center, 2017, Vol 24, Issue 4, p381

1300-1744

Academic Journal

10.5455/jtomc.2017.05.068