Title

Cytotoxic and antiviral potentials of Euphorbia milii var. splendens leaf against Peste des petits ruminant virus.

Authors

Chaman, Sadia; Khan, Farrakh Zia; Khokhar, Rabia; Maab, Husnul; Qamar, Shaista; Zahid, Sahar; Ahmad, Mobasher; Hussain, Khalid

Abstract

Purpose: To determine the cytotoxic and antiviral potentials of Euphorbia milii var. splendens leaf against Peste des petits ruminants virus (PPRV). Methods: The methanol extract, as well as n-hexane, chloroform, ethyl acetate, and n-butanol fractions of E. milii leaves were screened for cytotoxic and antiviral activities against PPRV using Vero cell line and 3-(4,5-dimethylthiazol-2-yl)-2, diphenyltetrazolium bromide (MTT) assay. Results: Non-cytotoxic concentrations with cell survival rate (CSP) greater than 50 % were considered virucidal. Methanol extract and fractions produced significant (p < 0.05) effects at all test concentrations against PPRV. The 50 % cytotoxic concentration (CC50) calculated was ≤ 25 µg/mL for extract and fractions. In antiviral assay, ethyl acetate, n-hexane, and n-butanol fractions at all test concentrations ranging from 1.56 - 800 µg/mL were non-virucidal; even at their non-cytotoxic concentrations these fractions did not show antiviral activities. However, the methanol extract and its chloroform fractions showed significant (p < 0.05) virucidal potential. Conclusion: The results suggest that further isolation of antiviral constituents from the fractions may open new horizons for the development of new antiviral agents.

Subjects

PESTE des petits ruminants; HEXANE; ETHYL acetate; EUPHORBIA

Publication

Tropical Journal of Pharmaceutical Research, 2019, Vol 18, Issue 7, p1507

ISSN

1596-5996

Publication type

Academic Journal

DOI

10.4314/tjpr.v18i7.21