We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Detection of an altered heterochromatin structure in the absence of the nucleotide excision repair protein Rad4 in Saccharomyces cerevisiae.
- Authors
Zhang, Ling; Chen, Hua; Bi, Xin; Gong, Feng
- Abstract
Rad4p is a DNA damage recognition protein essential for global genomic nucleotide excision repair in Saccharomyces cerevisiae. Here, we show that Rad4p binds to the heterochromatic HML locus. In a yeast mutant lacking Rad4p, an increased level of SIR complex binding at the HML locus is accompanied by an altered, more compact heterochromatin structure, as revealed by a topological analysis of chromatin circles released from the locus. In addition, gene silencing at the HML locus is enhanced in the rad4Δ mutant. Importantly, re-expression of Rad4p in the rad4Δ mutant restores the altered heterochromatin structure to a conformation similar to that detected in wild-type cells. These findings reveal a novel role of Rad4p in the regulation of heterochromatin structure and gene silencing.
- Publication
Cell cycle (Georgetown, Tex.), 2013, Vol 12, Issue 15, p2435
- ISSN
1551-4005
- Publication type
Journal Article
- DOI
10.4161/cc.25457