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- Title
Simultaneous determination of five marker constituents in Ssanghwa tang by HPLC/DAD.
- Authors
Jin Bae Won; Jin Yeul Ma; Young Ran Um; Choong Je Ma
- Abstract
A HPLC-DAD method was established for the simultaneous evaluation of five bioactive compounds in Ssanghwa tang (SHT) including glycyrrhizin, paeoniflorin, cinnamic acid, decursin and 6-gingerol. These compounds were separated in less than 40 min using a Dionex C18 column with a gradient elution system of water and methanol at a flow rate of 1 ml/min. Calibration curve of standard components presented excellent linear regression (R²>0.9903) within the test range. Limit of detection and limit of quantification varied from 0.07 to 0.46 μg/ml and 0.13 to 1.11 μg/ml, respectively. The relative standard deviations (RSDs) of data of the intraday and interday experiments were less than 3.67 and 5.73%, respectively. The accuracy of recovery test ranged from 95.98 to 105.88% with RSD values 0.10- 4.82%.
- Publication
Pharmacognosy Magazine, 2010, Vol 6, Issue 22, p111
- ISSN
0973-1296
- Publication type
Academic Journal
- DOI
10.4103/0973-1296.62896