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- Title
Nimesulide inhibits the proliferation of HepG2 by up-regulation of Smad4.
- Authors
Shaoqi Yang; Ruifang Guo; Liya Huang; Li Yang; Dong Jiang
- Abstract
Objectives: Hepatocellular carcinoma (HCC) is receiving increased attention. This study was designed to investigate the effect of selective Cyclooxygenase-2 (COX-2) inhibitor, nimesulide, on the expression of Smad4 in human hepatocellular carcinoma HepG2. Materials and Methods: HepG2 cells were incubated in various concentrations of nimesulide (25, 50, 100, 200, 400 μmol/L) to detect the effect of proliferation by MTS. The apoptosis of HepG2 was determined by TUNEL; fluorescence microscope was used to observe the expression of Smad4. Results: The result showed that nimesulide inhibited the proliferation of HepG2 cell in a concentrations-dependent manner, and promoted the karyopyknosis and fragmentation of HepG2 cell nucleus, induced its apoptosis, the number of fluorescence labeling of Smad4 in Nimesulide group was higher than control group ( P<0.05). Conclusions: Nimesulide inhibits the proliferation and promotes apoptosis of HepG2 by up-regulation of Smad4 in HepG2.
- Publication
Indian Journal of Pharmacology, 2012, Vol 44, Issue 5, p599
- ISSN
0253-7613
- Publication type
Academic Journal
- DOI
10.4103/0253-7613.100384