Bioinformatic Analysis of the Main Characteristics and Structure of the Gene Encoding CYP2E1 Protein.

Lin Fei-fan; Han Ya-li

The nucleotide and amino acid sequences of CYPPE1 protein (GenBank accession number; NC000010. 11 and NM000773. 3) from human being have been analyzed and predicted by bioinformatics in the following aspects ; physicochemical properties, hydrophilicity and hydrophobicity, the signal peptide, transmembrane topological structure, subcellular localization, protein secondary structure and tertiary structure, the enzyme active center structure, and the composition of homologous protein and phylogenetic relationship of human being and other 6 species. The results show that the CYPPE1 gene encodes a 493 amino acid polypeptides with the estimated relative molecular weight 172 386. 6, the theoretical PI of 7. 04 and the structural formula of C6778H10986N2074O2429S374;. It is also found that CYPPE1 is a transmembrane protein with hydrophobicity and relatively unstable. There are 6 ß fold, 6 a-helix, 6 transmembrane regions and 32 angles in its secondary structure, and Its active center was composed of Asn202 residues, Ser298 residues, Phe205 residues and iron porphyrin ring. The distance of its heme and substrate catalytic sites is 0.3-0.6 nm.

Journal of Guangdong University of Technology, 2015, Vol 32, Issue 1, p16

1007-7162

Academic Journal

10.3969/j.issn.1007-7162.2015.01.004