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- Title
Homozygous mutations in a predicted endonuclease are a novel cause of congenital dyserythropoietic anemia type I.
- Authors
Babbs, Christian; Roberts, Nigel A; Sanchez-Pulido, Luis; McGowan, Simon J; Ahmed, Momin R; Brown, Jill M; Sabry, Mohamed A; Bentley, David R; McVean, Gil A; Donnelly, Peter; Gileadi, Opher; Ponting, Chris P; Higgs, Douglas R; Buckle, Veronica J; WGS500 Consortium
- Abstract
The congenital dyserythropoietic anemias are a heterogeneous group of rare disorders primarily affecting erythropoiesis with characteristic morphological abnormalities and a block in erythroid maturation. Mutations in the CDAN1 gene, which encodes Codanin-1, underlie the majority of congenital dyserythropoietic anemia type I cases. However, no likely pathogenic CDAN1 mutation has been detected in approximately 20% of cases, suggesting the presence of at least one other locus. We used whole genome sequencing and segregation analysis to identify a homozygous T to A transversion (c.533T>A), predicted to lead to a p.L178Q missense substitution in C15ORF41, a gene of unknown function, in a consanguineous pedigree of Middle-Eastern origin. Sequencing C15ORF41 in other CDAN1 mutation-negative congenital dyserythropoietic anemia type I pedigrees identified a homozygous transition (c.281A>G), predicted to lead to a p.Y94C substitution, in two further pedigrees of SouthEast Asian origin. The haplotype surrounding the c.281A>G change suggests a founder effect for this mutation in Pakistan. Detailed sequence similarity searches indicate that C15ORF41 encodes a novel restriction endonuclease that is a member of the Holliday junction resolvase family of proteins.
- Publication
Haematologica, 2013, Vol 98, Issue 9, p1383
- ISSN
1592-8721
- Publication type
Journal Article
- DOI
10.3324/haematol.2013.089490