In vitro Plant Development of Aerides Multiflora Roxb. and Genetic Fidelity Assessment through RAPD Marker.

Paramanik, Purnima; Raha, Subrata; Kar, Dipak Kumar

Aerides multiflora Roxb., also known as the Fox Tail Orchid, is a rare epiphytic orchid of the Purulia District of West Bengal with ornamental and therapeutic values. The plant's wild populations have decreased due to limited natural regeneration and unrestricted commercial harvesting. Orchid seed germination relies on mycorrhizal symbiotic interaction. Our current research is focused on studying the germination of Aerides multiflora seeds and plantlet development using aseptic techniques to address the reduced natural regeneration process. This aseptic technique involves using Murashige and Skoog (MS) basal media with activated charcoal (AC) at different concentrations for seed germination, along with 6-Benzylaminopurine (BAP) and Naphthalene acetic acid (NAA) at varying concentrations for seedling development. The germination and protocorm formation varied widely, with the highest response (80.89 per cent) observed in the medium supplemented with 750 mg/l AC and the lowest (23.75 per cent) in the basal media. Most combinations tested showed variations in leaf and root formation and the percentages of multiple protocorm formation. Notably, MS medium with 750mg/l AC as the control, containing BAP 1.5 mg/l and NAA 0.5 mg/l, exhibited high percentages of leaf formation (90.5 per cent) and root formation (86.23 per cent). The plants were shifted to ex-vitro conditions and acclimatized with an 86.4 per cent survival rate. The in vitro grown plants were found to be identical clones of their parent plant with no signs of variation, as revealed by a Random Amplified Polymorphic DNA (RAPD) assessment. These findings will be helpful for the conservation and contribute to fulfil the demand of the floriculture and pharmaceutical industry through large-scale production.

RAPD technique; PLANT development; SEED development; ROOT formation; FLORICULTURE industry

Journal of Plant Science Research, 2024, Vol 40, Issue 3, p418

0970-2539

Academic Journal

10.32381/JPSR.2024.40.02.8