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- Title
Next-generation sequencing of Coccidioides immitis isolated during cluster investigation.
- Authors
Engelthaler, David M; Chiller, Tom; Schupp, James A; Colvin, Joshua; Beckstrom-Sternberg, Stephen M; Driebe, Elizabeth M; Moses, Tracy; Tembe, Waibhav; Sinari, Shripad; Beckstrom-Sternberg, James S; Christoforides, Alexis; Pearson, John V; Carpten, John; Keim, Paul; Peterson, Ashley; Terashita, Dawn; Balajee, S Arunmozhi
- Abstract
Next-generation sequencing enables use of whole-genome sequence typing (WGST) as a viable and discriminatory tool for genotyping and molecular epidemiologic analysis. We used WGST to confirm the linkage of a cluster of Coccidioides immitis isolates from 3 patients who received organ transplants from a single donor who later had positive test results for coccidioidomycosis. Isolates from the 3 patients were nearly genetically identical (a total of 3 single-nucleotide polymorphisms identified among them), thereby demonstrating direct descent of the 3 isolates from an original isolate. We used WGST to demonstrate the genotypic relatedness of C. immitis isolates that were also epidemiologically linked. Thus, WGST offers unique benefits to public health for investigation of clusters considered to be linked to a single source.
- Publication
Emerging infectious diseases, 2011, Vol 17, Issue 2, p227
- ISSN
1080-6059
- Publication type
Journal Article
- DOI
10.3201/eid1702.100620