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- Title
European bat lyssavirus in Scottish bats.
- Authors
Brookes, Sharon M; Aegerter, James N; Smith, Graham C; Healy, Derek M; Jolliffe, Tracey A; Swift, Susan M; Mackie, Iain J; Pritchard, J Stewart; Racey, Paul A; Moore, Niall P; Fooks, Anthony R
- Abstract
We report the first seroprevalence study of the occurrence of specific antibodies to European bat lyssavirus type 2 (EBLV-2) in Daubenton's bats. Bats were captured from 19 sites across eastern and southern Scotland. Samples from 198 Daubenton's bats, 20 Natterer's bats, and 6 Pipistrelle's bats were tested for EBLV-2. Blood samples (N = 94) were subjected to a modified fluorescent antibody virus neutralization test to determine antibody titer. From 0.05% to 3.8% (95% confidence interval) of Daubenton's bats were seropositive. Antibodies to EBLV-2 were not detected in the 2 other species tested. Mouth swabs (N = 218) were obtained, and RNA was extracted for a reverse transcription-polymerase chain reaction (RT-PCR). The RT-PCR included pan lyssavirus-primers (N gene) and internal PCR control primers for ribosomal RNA. EBLV-2 RNA was not detected in any of the saliva samples tested, and live virus was not detected in virus isolation tests.
- Publication
Emerging infectious diseases, 2005, Vol 11, Issue 4, p572
- ISSN
1080-6040
- Publication type
Journal Article
- DOI
10.3201/eid1104.040920