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- Title
Longitudinally profiling neutralizing antibody response to SARS coronavirus with pseudotypes.
- Authors
Temperton, Nigel J; Chan, Paul K; Simmons, Graham; Zambon, Maria C; Tedder, Richard S; Takeuchi, Yasuhiro; Weiss, Robin A
- Abstract
The severe acute respiratory syndrome-associated coronavirus (SARS-CoV) spike protein (S) is a major target for neutralizing antibodies. Retroviral SARS-CoV S pseudotypes have been constructed and used to develop an in vitro microneutralization assay that is both sensitive and specific for SARS-CoV neutralizing antibodies. Neutralization titers measured by this assay are highly correlated to those measured by an assay using replication-competent SARS-CoV. No cross-neutralization occurred with human sera known to contain antibodies to coronavirus strains OC43 and 229E. The pseudotype assay was used to profile neutralizing antibody responses against SARS-CoV S in sequential serum samples taken from 41 confirmed SARS patients during the 2003 outbreak in Hong Kong and shows long-lasting immunity in most recovered patients. The pseudotype assay does not require handling live SARS virus; it is a useful tool to determine neutralizing titers during natural infection and the preclinical evaluation of candidate vaccines.
- Publication
Emerging infectious diseases, 2005, Vol 11, Issue 3, p411
- ISSN
1080-6040
- Publication type
Journal Article
- DOI
10.3201/eid1103.040906