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- Title
Real-time reverse transcription-polymerase chain reaction assay for SARS-associated coronavirus.
- Authors
Emery, Shannon L; Erdman, Dean D; Bowen, Michael D; Newton, Bruce R; Winchell, Jonas M; Meyer, Richard F; Tong, Suxiang; Cook, Byron T; Holloway, Brian P; McCaustland, Karen A; Rota, Paul A; Bankamp, Bettina; Lowe, Luis E; Ksiazek, Tom G; Bellini, William J; Anderson, Larry J
- Abstract
A real-time reverse transcription-polymerase chain reaction (RT-PCR) assay was developed to rapidly detect the severe acute respiratory syndrome-associated coronavirus (SARS-CoV). The assay, based on multiple primer and probe sets located in different regions of the SARS-CoV genome, could discriminate SARS-CoV from other human and animal coronaviruses with a potential detection limit of <10 genomic copies per reaction. The real-time RT-PCR assay was more sensitive than a conventional RT-PCR assay or culture isolation and proved suitable to detect SARS-CoV in clinical specimens. Application of this assay will aid in diagnosing SARS-CoV infection.
- Publication
Emerging infectious diseases, 2004, Vol 10, Issue 2, p311
- ISSN
1080-6040
- Publication type
Journal Article
- DOI
10.3201/eid1002.030759