Title

The Effect of Crab Extract on the proliferation Activity of Colon Cancer Cell Line HCT-116.

Authors

KADHUM, ALI RASIM; AL-MUKHTAR, ENTISAR JAWAD; GHALEB, RANA A.; OBIED, HAMID NAJI

Abstract

Background: Colon cancer is the fifth most common type of malignant growth in the world. Although treatment of cancer have good clinical outcome, chemotherapy used in treatment of cancer is still required more development. In traditional medicine, crab (Brachyura) is recommended for treatment of cancer. Objective: Investigate the in-vitro cytotoxic effect of crab chloroform extract on colon HCT-116 cell line and normal cell line (MDCK). Material and Methods: Crab chloroform extract was prepared in many concentrations (31.25, 62.5, 125, 250, 500, 1000 µg/mL) and assessed on HCT and MDCK cell lines over two period of incubation (24 and 48 hours) by MTT assay. Data were analyzed using a one-sample test by SPSS (version 25) one way Anova computer program and P0.05) affected by crab extract of both incubation periods (24 and 48 hours) except in high dose 1000 µg/mL after 48 hours was significant (P<0.05). Conclusion: The decrease in cell viability of colon cancer cells and unaffected normal cells reveal that the crab extract has a selective anti-proliferative effect.

Subjects

COLON cancer; CELL lines; CRABS; CANCER treatment; EXTRACTS

Publication

International Journal of Pharmaceutical Research (09752366), 2020, p1741

ISSN

0975-2366

Publication type

Academic Journal

DOI

10.31838/ijpr/2020.SP1.268