We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Generation and functional analysis of monoclonal antibodies against the second extracellular loop of human M3 muscarinic acetylcholine receptor.
- Authors
Tsuboi, Hiroto; Nakamura, Yumi; Iizuka, Mana; Matsuo, Naomi; Matsumoto, Isao; Sumida, Takayuki
- Abstract
The M3 muscarinic acetylcholine receptor (M3R) plays a crucial role in the activation of salivary and lachrymal glands. The M3R contains four extracellular domains (the N-terminal, and the first, second, and third extracellular loops), and we recently detected antibodies against each of these four domains in a subgroup of patients with Sjögren's syndrome (SS). Functional analysis indicated that the influence of such anti-M3R antibodies on salivary secretion might differ based on the epitopes to which they bind. To clarify the relationship between B-cell epitopes on the M3R and its function, we generated two hybridomas producing anti-M3R monoclonal antibodies against the second extracellular loop of M3R (anti-M3R mAbs) and analyzed their function by Ca-influx assays, using a human salivary gland (HSG) cell line. These two anti-M3R mAbs suppressed Ca-influx in the HSG cells induced by cevimeline stimulation, suggesting that autoantibodies against the second extracellular loop of M3R could be involved in salivary dysfunction in patients with SS.
- Publication
Modern Rheumatology, 2012, Vol 22, Issue 2, p264
- ISSN
1439-7595
- Publication type
Academic Journal
- DOI
10.3109/s10165-011-0514-8