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- Title
Very slow turnover of beta-cells in aged adult mice.
- Authors
Teta, Monica; Long, Simon Y; Wartschow, Lynn M; Rankin, Matthew M; Kushner, Jake A
- Abstract
Although many signaling pathways have been shown to promote beta-cell growth, surprisingly little is known about the normal life cycle of preexisting beta-cells or the signaling pathways required for beta-cell survival. Adult beta-cells have been speculated to have a finite life span, with ongoing adult beta-cell replication throughout life to replace lost cells. However, little solid evidence supports this idea. To more accurately measure adult beta-cell turnover, we performed continuous long-term labeling of proliferating cells with the DNA precursor analog 5-bromo-2-deoxyuridine (BrdU) in 1-year-old mice. We show that beta-cells of aged adult mice have extremely low rates of replication, with minimal evidence of turnover. Although some pancreatic components acquired BrdU label in a linear fashion, only 1 in approximately 1,400 adult beta-cells were found to undergo replication per day. We conclude that adult beta-cells are very long lived.
- Publication
Diabetes, 2005, Vol 54, Issue 9, p2557
- ISSN
0012-1797
- Publication type
Journal Article
- DOI
10.2337/diabetes.54.9.2557