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- Title
In vitro-deranged intestinal immune response to gliadin in type 1 diabetes.
- Authors
Auricchio, Renata; Paparo, Francesco; Maglio, Maria; Franzese, Adriana; Lombardi, Francesca; Valerio, Giuliana; Nardone, Gerardo; Percopo, Selvaggia; Greco, Luigi; Troncone, Riccardo
- Abstract
Dietary gluten has been associated with an increased risk of type 1 diabetes. We have evaluated inflammation and the mucosal immune response to gliadin in the jejunum of patients with type 1 diabetes. Small intestinal biopsies from 17 children with type 1 diabetes without serological markers of celiac disease and from 50 age-matched control subjects were examined by immunohistochemistry. In addition, biopsies from 12 type 1 diabetic patients and 8 control subjects were cultured with gliadin or ovalbumin peptic-tryptic digest and examined for epithelial infiltration and lamina propria T-cell activation. The density of intraepithelial CD3(+) and gammadelta(+) cells and of lamina propria CD25(+) mononuclear cells was higher in jejunal biopsies from type 1 diabetic patients versus control subjects. In the patients' biopsies cultured with peptic-tryptic gliadin, there was epithelial infiltration by CD3(+) cells, a significant increase in lamina propria CD25(+) and CD80(+) cells and enhanced expression of lamina propria CD54 and crypt HLA-DR. No such phenomena were observed in control subjects, even those with celiac disease-associated HLA haplotypes. In conclusion, signs of mucosal inflammation were present in jejunal biopsies from type 1 diabetic patients, and organ culture studies indicate a deranged mucosal immune response to gliadin.
- Publication
Diabetes, 2004, Vol 53, Issue 7, p1680
- ISSN
0012-1797
- Publication type
Journal Article
- DOI
10.2337/diabetes.53.7.1680