Title

Antibiotic production by Streptomyces hygroscopicus CH-7 in medium containing Schiff base complexes.

Authors

Ilić, Slavica B.; Konstantinović, Sandra S.; Cvijović, Gordana D. Gojgić; Veljković, Vlada B.

Abstract

Influence of a modified media on Hexaene H-85 and Azalomycine B production by Streptomyces hygroscopicus CH-7 was investigated. The amino acid L-tryptophan, as a nitrogen source, was replaced with isatin-3-thiosemicarbazone and its complexes with some divalent metal ions. Isatin-3-thiosemicarbazone was synthesized in crude glycerol obtained as a byproduct in biodiesel production from sunflower oil. The complexes were characterized by elemental microanalysis and magnetic susceptibility, as well as, by Atomic absorption(AA), Fourier-transform infrared (FTIR) and Ultraviolet-visible (UV/VIS) spectroscopic methods. The spectral studies indicated an octahedral geometry for the Mn(II), Fe(II) and Ni(II) complexes and a tetrahedral one for the Zn(II) complex. Comparing to the basal medium, isatin-3-thiosemicarbazone (ITC) and its metal complexes in the concentration of 0.5 g dm-3 showed better results in the antibiotics production. Use of medium supplemented with the Fe(II) complex resulted in the maximum Hexaene H-85 and Azalomycine B concentrations of 306 μg cm-3 and 127 μg cm-3, respectively. Addition of ITC and its complexes changed the morphology of S. hygroscopicus CH-7 from filaments to pellets as a dominant shape in media resulting in higher antibiotic production.

Subjects

SCHIFF bases; STREPTOMYCES; SUNFLOWER seed oil; GLYCERIN; MAGNETIC susceptibility; MASS media use; TRYPTOPHAN

Publication

Chemical Industry / Hemijska Industrija, 2019, Vol 73, Issue 2, p93

ISSN

0367-598X

Publication type

Academic Journal

DOI

10.2298/HEMIND181016008I