Title

Scanning Electron Microscopy Analysis of Tea's Embryo Axis Explant Cultured on Murashige and Skoog Medium Containing 2,4-Dichlorophenoxyacetic acid.

Authors

Eskundari, Ratna Dewi; Taryono; Indradewa, Didik; Purwestri, Yekti Asih

Abstract

Camellia sinensis L. is an important crop in Indonesia as healthy beverage that contains several secondary metabolism compounds, such as polyphenols and catechins. Tissue culture including somatic embryogenesis and organogenesis has been used for propagating plant for various needs. In this present shortcommunication, scanning electron microscopic (SEM) analysis of tea was conducted and discussed. This study aimed to investigate surface ultrastructure of TRI2025 embryo axis tea clone cultured on Murashige and Skoog (MS) medium containing 2,4-Dichlorophenoxyacetic acid (2,4-D). The results revealed two different forms of explant's development, i.e. somatic embryo and transitional form between somatic embryogenesis and organogenesis; or called by "Globular-like Structure" (GLS). Surface ultrastructure analys is of somatic embryo and GLS revealed respect ively many stages of somatic embryo development i.e. globular, torpedo, and cotyledon stage, and leaf development form GLS regeneration.

Subjects

SCANNING electron microscopy; TEA; SECONDARY metabolism; MORPHOGENESIS; SOMATIC embryogenesis

Publication

Journal of Tropical Biodiversity & Biotechnology, 2024, Vol 9, Issue 1, p1

ISSN

2540-9573

Publication type

Academic Journal

DOI

10.22146/jtbb.76451