Phosphodiesterase inhibitor Theophylline in the aged laying hens: investigation of the ovarian and fallopian inflammatory mediators, hormones, and functions.

Pirsaraei, Zarbakht Ansari; Hatefi, Ali; Deldar, Hamid; Shahneh, Ahmad Zare

Inflammatory-dependent events in the aged commercial laying hens reduce production rate and egg quality. Our study was aimed to evaluate the phosphodiesterase (PDE) inhibitor Theophylline on the ovarian and fallopianinflammatory traits in the commercial laying hens at the late stage of the production period. Twenty-four White Leghorn hens aged 92 weeks were used for four weeks to be orally supplemented by Theophylline (3 mg/kg/Body weight, BW). Ovulation rate and follicular growth were measured by laying frequency and, visual evaluation after euthanizing, respectively. The mRNA expressions of follicular and fallopian cyclooxygenases 1 and 2 (COX-1,-2), and cytokines were detected by real-time PCR. Plasma concentrations of ovarian hormones (Two hours after lighting) were measured via enzyme-linked immunosorbant assay (ELISA). The results showed that the mRNA expression of the IL-1ß, IL-6 and TNF-a in the ovary and IL-1ß and TNF-a in the infundibulum were significantly decreased (P0.05) influenced by Theophylline. As a phosphodiesterase inhibitor, Theophylline retained the laying frequency in addition to enhancing its anti-inflammatory capability in the late stage of production period in laying hens.

HENS; PHOSPHODIESTERASE inhibitors; INFLAMMATORY mediators; CYCLOOXYGENASES; POLYMERASE chain reaction; THEOPHYLLINE; EGG quality; ANIMAL herds

Journal of Livestock Science & Technologies (JLST), 2023, Vol 11, Issue 1, p43

2322-3553

Academic Journal

10.22103/JLST.2023.20605.1446