We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
SNP-Ratio Mapping (SRM): identifying lethal alleles and mutations in complex genetic backgrounds by next-generation sequencing.
- Authors
Lindner, Heike; Raissig, Michael T; Sailer, Christian; Shimosato-Asano, Hiroko; Bruggmann, Rémy; Grossniklaus, Ueli
- Abstract
We present a generally applicable method allowing rapid identification of causal alleles in mutagenized genomes by next-generation sequencing. Currently used approaches rely on recovering homozygotes or extensive backcrossing. In contrast, SNP-ratio mapping allows rapid cloning of lethal and/or poorly transmitted mutations and second-site modifiers, which are often in complex genetic/transgenic backgrounds.
- Publication
Genetics, 2012, Vol 191, Issue 4, p1381
- ISSN
1943-2631
- Publication type
Journal Article
- DOI
10.1534/genetics.112.141341