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- Title
Targeting DNA double-strand breaks with TAL effector nucleases.
- Authors
Christian, Michelle; Cermak, Tomas; Doyle, Erin L; Schmidt, Clarice; Zhang, Feng; Hummel, Aaron; Bogdanove, Adam J; Voytas, Daniel F
- Abstract
Engineered nucleases that cleave specific DNA sequences in vivo are valuable reagents for targeted mutagenesis. Here we report a new class of sequence-specific nucleases created by fusing transcription activator-like effectors (TALEs) to the catalytic domain of the FokI endonuclease. Both native and custom TALE-nuclease fusions direct DNA double-strand breaks to specific, targeted sites.
- Publication
Genetics, 2010, Vol 186, Issue 2, p757
- ISSN
1943-2631
- Publication type
Journal Article
- DOI
10.1534/genetics.110.120717