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Title
A simple polymerase chain reaction-based method for the construction of recombinase-mediated cassette exchange donor vectors.
Authors
Bateman, Jack R; Wu, C-ting
Abstract
Here we describe a simple method for generating donor vectors suitable for targeted transgenesis via recombinase-mediated cassette exchange (RMCE) using the PhiC31 integrase. This PCR-based strategy employs small attB "tails" on the primers used to amplify a sequence of interest, permitting the rapid creation of transgenes for in vivo analysis.