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- Title
Completely Distinguishing Individual A-Genome Chromosomes and Their Karyotyping Analysis by Multiple Bacterial Artificial Chromosome-Fluorescence in Situ Hybridization.
- Authors
Kai Wang; Bing Guan; Wangzhen Guo; Baoliang Zhou; Yan Hu; Yichao Zhu; Tianzhen Zhang
- Abstract
Twenty bacterial artificial chromosome (BAG) clones that could produce bright signals and no or very low fluorescence in situ hybridization (FISH) background were identified from Gossypium arboreum cv. JLZM, and G. hirsutum accession (acc.) TM-1 and 0-613-2R. Combining with 45S and 5S rDNA, a 22-probe cocktail that could identify all 13 G. arboreum chromosomes simultaneously was developed. According to their homology with tetraploid cotton, the G. arboreum chromosomes were designated as A1-A13, and a standard karyotype analysis of G. arboreum was presented. These results demonstrated an application for multiple BAC-FISH in cotton cytogenetic studies and a technique to overcome the problem of simultaneous chromosome recognition in mitotic cotton cells.
- Publication
Genetics, 2008, Vol 178, Issue 2, p1117
- ISSN
0016-6731
- Publication type
Academic Journal
- DOI
10.1534/genetics.107.083576