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- Title
Saccharomyces cerevisiae Sae2- and Tell-Dependent Single-Strand DNA Formation at DNA Break Promotes Microhomology-Mediated End Joining.
- Authors
Kihoon Lee; Sang Eun Lee
- Abstract
Microhomology-mediated end joining (MMEJ) joins DNA ends via short stretches [5-20 nucleoticles (nt)] of direct repeat sequences, yielding deletions of intervening sequences. Non-homologous end joining (NHEJ) and single-strand annealing (SSA) are other error prone processes that anneal single-stranded DNA (ssDNA) via a few bases (<5 nt) or extensive direct repeat homologies (>20 nt). Although the genetic components involved in MMEJ are largely unknown, those in NHEJ anti SSA are characterized in some detail. Here, we surveyed the role of NHEJ or SSA factors in joining of double-strand breaks (DSBs) with no complementary DNA ends that rely primarily on MMEJ repair. We found that MMEJ requires the nuclease activity of Mrell/Rad50/Xrs2, 3′ flap removal by Rad1/Rad10, Nej1, and DNA synthesis by multiple polymerases including PoI4, Racl30, Rev3, and Pol32. The mismatch repair proteins, Rad52 group genes, and Rad27 are dispensable for MMEJ. Sae2 and Tell promote MMEJ but inhibit NHEJ, likely by regulating Mre11-dependent ssDNA accumulation at DNA break. Our data support the role of Sae2 and Tell in MMEJ and genome integrity.
- Publication
Genetics, 2007, Vol 176, Issue 4, p2003
- ISSN
0016-6731
- Publication type
Academic Journal
- DOI
10.1534/genetics.107.076539