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- Title
A chromosome bin map of 16,000 expressed sequence tag loci and distribution of genes among the three genomes of polyploid wheat.
- Authors
Qi, L L; Echalier, B; Chao, S; Lazo, G R; Butler, G E; Anderson, O D; Akhunov, E D; Dvorák, J; Linkiewicz, A M; Ratnasiri, A; Dubcovsky, J; Bermudez-Kandianis, C E; Greene, R A; Kantety, R; La Rota, C M; Munkvold, J D; Sorrells, S F; Sorrells, M E; Dilbirligi, M; Sidhu, D; Erayman, M; Randhawa, H S; Sandhu, D; Bondareva, S N; Gill, K S; Mahmoud, A A; Ma, X-F; Miftahudin; Gustafson, J P; Conley, E J; Nduati, V; Gonzalez-Hernandez, J L; Anderson, J A; Peng, J H; Lapitan, N L V; Hossain, K G; Kalavacharla, V; Kianian, S F; Pathan, M S; Zhang, D S; Nguyen, H T; Choi, D-W; Fenton, R D; Close, T J; McGuire, P E; Qualset, C O; Gill, B S
- Abstract
Because of the huge size of the common wheat (Triticum aestivum L., 2n = 6x = 42, AABBDD) genome of 17,300 Mb, sequencing and mapping of the expressed portion is a logical first step for gene discovery. Here we report mapping of 7104 expressed sequence tag (EST) unigenes by Southern hybridization into a chromosome bin map using a set of wheat aneuploids and deletion stocks. Each EST detected a mean of 4.8 restriction fragments and 2.8 loci. More loci were mapped in the B genome (5774) than in the A (5173) or D (5146) genomes. The EST density was significantly higher for the D genome than for the A or B. In general, EST density increased relative to the physical distance from the centromere. The majority of EST-dense regions are in the distal parts of chromosomes. Most of the agronomically important genes are located in EST-dense regions. The chromosome bin map of ESTs is a unique resource for SNP analysis, comparative mapping, structural and functional analysis, and polyploid evolution, as well as providing a framework for constructing a sequence-ready, BAC-contig map of the wheat genome.
- Publication
Genetics, 2004, Vol 168, Issue 2, p701
- ISSN
0016-6731
- Publication type
Journal Article
- DOI
10.1534/genetics.104.034868