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- Title
SepB<sup>CTF4</sup> Is Required for the Formation of DNA-Damage-Induced UvsC<sup>RAD51</sup> Foci in Aspergillus nidulans.
- Authors
Gygax, Scott E.; Semighini, Camile P.; Goldman, Gustavo H.; Harris, Steven D.
- Abstract
SepB is an essential, conserved protein required for chromosomal DNA metabolism in Aspergillus nidulans. Homologs of SepB include yeast Ctf4p and human hAnd-1. Molecular and bioinformatic characterization of these proteins suggests that they act as molecular scaffolds. Furthermore, recent observations implicate the yeast family members in lagging-strand replication and the establishment of sister-chromatid cohesion. Here, we demonstrate that SepB functions in the A. nidulans DNA damage response. In particular, analysis of double mutants reveals that SepB is a member of the UvsCRAD51 epistasis group. In accord with this prediction, we show that UvsCRAD51 forms DNA-damage-induced nuclear foci in a manner that requires SepB function. We also provide evidence that implicates SepB in sister-chromatid cohesion, thereby suggesting that cohesion may play a role in regulating tile localization and/or assembly of UvsCRAD51 complexes.
- Publication
Genetics, 2005, Vol 169, Issue 3, p1391
- ISSN
0016-6731
- Publication type
Academic Journal
- DOI
10.1534/genetics.104.030817