Amelioration of Hepato-Renal Toxicity by a Flavonoid-Rich Fraction of Hibiscus rosa-sinenses (Leaves and Flowers) in Male Rabbits Intoxicated by Cadmium.

Hussain, Naqi; Chaudhary, Muhammad Nawaz; Anjum, Aftab Ahmed; Abbas, Naeem; Zaheer, Muhammad; Mustafa, Ghulam; Majid, Muzaffar

This study was initiated to anticipate the potential protective effects of flavonoid-rich fractions of Hibiscus rosa-sinenses flowers (HF) and leaves (HL) on cadmium (Cd)-induced toxicity in adult albino rabbits. Cd, being a prominent environmental pollutant, poses a threat for its widespread range of toxic expressions. A dramatic increase in total phenolics, flavonoids, and antioxidant capacity has been observed in flavonoid-rich fractions of HF and HL as compared to their crude extracts, suggesting for the rationale for their use in this study. Thirty-six male rabbits were indiscriminately allocated into six groups, including control, Cd (6.1 mg Cd/kg/day), HF and HL (200 mg HF & HL /Kg/day), Cd HF, and Cd HL (receiving the same doses of Cd, HF, and HL) groups. Treatment (p.o) for 60 days resulted in improvement of weight gain in Cd HF and Cd HL groups in comparison with the Cd group. Treatment with Cd HF showed better results than Cd HL by significantly decreasing (pth, 30th, 45th, and 60th days in a progressive manner when compared with the Cd receiving group. Likewise, the Cd HF group showed better activities than Cd HL by significantly increasing (p<0.05) superoxide dismutase, catalase, and vitamins C and E, while LPO and Cd concentrations were significantly decreased in the liver and kidney tissues when compared with Cd-intoxicated bucks. Values of these parameters in only HF and HL-receiving groups were near the control. The histopathological studies for these tissues have also sustained the above-mentioned data. Thus, these outcomes suggest that the flavonoid-rich fraction of HF may act as a potent hepatic and renal protective agent than HL against Cd-induced toxicity in animal models.

NEPHROTOXICOLOGY; FLAVONOIDS; CADMIUM; GROUP 12 elements; POLLUTANTS; AMINOTRANSFERASES

Polish Journal of Environmental Studies, 2017, Vol 26, Issue 4, p1551

1230-1485

Academic Journal

10.15244/pjoes/68873