The protective roles of zinc and estradiol in renal ischemia/reperfusion injury in ovariectomized rats.

Barekat, Foroogh; Talebi, Ardeshir; Nematbakhsh, Mehdi

Background: One of the main events associated with ischemia/reperfusion injury (IRI) is excessive production of reactive oxygen species (ROS). Zinc (Zn) and estradiol are considered as antioxidants that scavenge free radicals. Objectives: The aim of this study was to compare the protective effect of Zn and estradiol against renal IRI in ovariectomized rats. Materials and Methods: Ovariectomized Wistar rats were randomly divided into five experimental groups including control (sham operated), IRI, estradiol treated IRI, estradiol and Zn treated IRI, Zn treated IRI groups. The IRI was induced by clamping renal vessels for 45 minutes followed by 24 hours reperfusion. During the last 6 hours of reperfusion, urine output was collected and the measurements were performed. Results: IRI caused an increase in kidney tissue damage score (KTDS) significantly (P < 0.05). The serum levels of blood urea nitrogen (BUN) and the creatinine (Cr) also elevated by IRI, but theses parameters attenuated by Zn treatment significantly (P < 0.05). Cr-clearance and urine flow were increased by Zn, and the percent of sodium excretion was increased by estradiol significantly (P < 0.05). The kidney tissue level of malondialdehyde was decreased by co-treatment of Zn and estradiol statistically (P < 0.05). Conclusions: Zn protected the kidney against IRI with an evident improvements of serum BUN and Cr levels, Cr clearance and tissue damage.

ESTRADIOL; ISCHEMIA; REPERFUSION injury

Journal of Nephropathology, 2018, Vol 7, Issue 2, p88

2251-8363

Academic Journal

10.15171/jnp.2018.21