Title

Wendlandia exserta: a pertinent source of antioxidant and antimicrobial agent.

Authors

Shahzadi, Tayyaba; Riaz, Tauheeda; Abbasi, Muhammad Athar; Mazhar, Farhana; Shahid, Muhammad; Ajaib, Muhammad

Abstract

Objective: The aim of the present investigation was to assess the antioxidant, antibacterial and antifungal activities of various fractions of Wendlandia exserta (Roxbukan Pansara). Methods: The methanolic extract of W. exserta (Roxbukan Pansara) was dissolved in water (distilled) and then partitioned with solvents e.g. n-hexane, CHCl3, EtOAc and n-BuOH consecutively. Antioxidant potential of above mentioned fractions and remaining aqueous fraction was checked by four methods. Antimicrobial activity was examined by four bacteria and four fungi by the disc diffusion method by using a standard, as positive control. Results: The aqueous fraction displayed maximum value of diphenyl picryl hydrazyl (DPPH) radical inhibition (85.94%±0.35) with an IC50 value of 415.31±0.26 μg mL−1. The aqueous fraction also displayed the maximum ferric reducing antioxidant power (FRAP) value [64.5±0.15 μg of trolox equivalents (TE)] as well as maximum inhibition of lipid peroxidation (42.32%±0.59) and maximum value of total phenolic contents (87.10±1.10). However, the n-BuOH fraction was found to display the maximum value of total antioxidant activity (0.529±0.76). The fractions also displayed good antimicrobial activity against various microorganisms. Conclusions: It was concluded that polar fractions of W. exserta showed good antioxidant and antimicrobial activity, so these might be useful in pharmacological preparations.

Subjects

RUBIACEAE; ANTIOXIDANTS; ANTI-infective agents; SOLVENTS; AQUEOUS solutions; THERAPEUTICS

Publication

Turkish Journal of Biochemistry / Turk Biyokimya Dergisi, 2018, Vol 43, Issue 4, p456

ISSN

0250-4685

Publication type

Academic Journal

DOI

10.1515/tjb-2017-0253