We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
A completed KLK activome profile: investigation of activation profiles of KLK9, 10, and 15.
- Authors
Hyesook Yoon; Blaber, Sachiko I.; Debela, Mekdes; Goettig, Peter; Scarisbrick, Isobel A.; Blaber, Michael
- Abstract
We previously reported the activation profiles of the human kallikrein-related peptidases (KLKs) as determined from a KLK pro-peptide fusion-protein system. That report described the activity profiles of 12 of the 15 mature KLKs versus the 15 different pro-KLK sequences. The missing profiles in the prior report, involving KLK9, 10, and 15, are now described. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis, mass spectrometry, and N-terminal sequence analyses show that KLK9 and 10 exhibit low hydrolytic activities towards all of the 15 pro-KLK sequences, while KLK15 exhibits significant activity towards both Arg- and Lys-containing KLK pro-sequences. The ability of KLK15 to activate pro-KLK8, 12, and 14 is confirmed using recombinant pro-KLK proteins, and shown to be significant for activation of pro-KLK8 and 14, but not 12. These additional data for KLK9, 10, and 15 now permit a completed KLK activome profile, using a KLK pro-peptide fusion-protein system, to be described. The results suggest that KLK15, once activated, can potentially feed back into additional pro-KLK activation pathways. Conversely, KLK9 and 10, once activated, are unlikely to participate in further pro-KLK activation pathways, although similar to KLK1 they may activate other bioactive peptides.
- Publication
Biological Chemistry, 2009, Vol 390, Issue 4, p373
- ISSN
1431-6730
- Publication type
Academic Journal
- DOI
10.1515/BC.2009.026